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Vascular endothelial growth factor (VEGF), BCL-2, and BAX expression in fibropapilloma tumor tissue and skin tissue of sea turtles
- Date Issued:
- 2009
- Summary:
- In sea turtles, the study of the etiology and development of fibropapillomatosis is not fully understood. Sea turtle fibropapillomatosis is a disease characterized by the proliferation of skin fibropapillomas and occasional internal fibromas. In this study, sea turtle fibropapilloma tumor and healthy tissue samples were used to look at VEGF, BCL-2 and Bax expression. Cancer tumors have a well established pattern of protein expression that involves overexpression of vascular endothelial growth factor (VEGF), responsible for the growth of new blood vessels, and a high BCL-2 to Bax ratio that leads to uncontrolled cell proliferation. Real time PCR was used to analyze VEGF expression, and Western blot techniques were used to measure BCL-2 and Bax expression. The results indicated that expression of VEGF was not significantly higher in tumor vs. skin tissue. For the differential expression of BCL-2 and Bax, the results were not in agreement with the established levels found in cancer studies, showing no significant change in BCL-2 expression and significantly higher levels of Bax in tumor vs. healthy tissue.
Title: | Vascular endothelial growth factor (VEGF), BCL-2, and BAX expression in fibropapilloma tumor tissue and skin tissue of sea turtles. |
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Name(s): |
Bancalari-Schmidlapp, Angela. Charles E. Schmidt College of Science Department of Biological Sciences |
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Type of Resource: | text | |
Genre: | Electronic Thesis Or Dissertation | |
Issuance: | multipart monograph | |
Date Issued: | 2009 | |
Publisher: | Florida Atlantic University | |
Physical Form: | electronic | |
Extent: | x, 58 p. : ill. (some col.). | |
Language(s): | English | |
Summary: | In sea turtles, the study of the etiology and development of fibropapillomatosis is not fully understood. Sea turtle fibropapillomatosis is a disease characterized by the proliferation of skin fibropapillomas and occasional internal fibromas. In this study, sea turtle fibropapilloma tumor and healthy tissue samples were used to look at VEGF, BCL-2 and Bax expression. Cancer tumors have a well established pattern of protein expression that involves overexpression of vascular endothelial growth factor (VEGF), responsible for the growth of new blood vessels, and a high BCL-2 to Bax ratio that leads to uncontrolled cell proliferation. Real time PCR was used to analyze VEGF expression, and Western blot techniques were used to measure BCL-2 and Bax expression. The results indicated that expression of VEGF was not significantly higher in tumor vs. skin tissue. For the differential expression of BCL-2 and Bax, the results were not in agreement with the established levels found in cancer studies, showing no significant change in BCL-2 expression and significantly higher levels of Bax in tumor vs. healthy tissue. | |
Identifier: | 369176266 (oclc), 209981 (digitool), FADT209981 (IID), fau:3006 (fedora) | |
Note(s): |
by Angela Bancalari-Schmidlapp. Thesis (M.S.)--Florida Atlantic University, 2009. Includes bibliography. Electronic reproduction. Boca Raton, Fla., 2009. Mode of access: World Wide Web. |
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Subject(s): |
Sea turtles -- Physiology Cancer -- Pathophysiology Vascular endothelial growth factors -- Pathophysiology Cellular signal transduction |
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Persistent Link to This Record: | http://purl.flvc.org/FAU/209981 | |
Use and Reproduction: | http://rightsstatements.org/vocab/InC/1.0/ | |
Host Institution: | FAU |