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GAD 65 and its role in pancreatic tissue survival
| Title: | GAD 65 and its role in pancreatic tissue survival. |
 84 views 13 downloads |
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| Name(s): |
Kumari, Neeta. Charles E. Schmidt College of Medicine Department of Biomedical Science |
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| Type of Resource: | text | |
| Genre: | Electronic Thesis Or Dissertation | |
| Date Issued: | 2012 | |
| Publisher: | Florida Atlantic University | |
| Physical Form: | electronic | |
| Extent: | ix, 37, [vi] p. : ill. (some col.) | |
| Language(s): | English | |
| Summary: | We employed three genotypes of GAD 65, wildtype (GAD 65 +/+), heterozygous (GAD 65 +/-) and knockout (GAD 65 -/-) to investigate the role of GAD 65 in survival of pancreatic islets. We analyzed the mRNA expression of pro-survival proteins including Bcl2 and Bax in pancreas of wildtype, heterozygous and knockout using Reverse Transcriptase Polymerase Chain Reaction (RTPCR). The level of expression of Bcl2 mRNA was down regulated in knockout mice pancreas and Bax to Bcl2 ratio was found higher in knockout mice pancreas suggesting higher cell death rate. However, further studies are required to recognize and understand the specific connections between apoptotic pathways and GAD 65 in pancreatic islets. | |
| Identifier: | 794671814 (oclc), 3342206 (digitool), FADT3342206 (IID), fau:3877 (fedora) | |
| Note(s): |
by Neeta Kumari. Thesis (M.S.)--Florida Atlantic University, 2012. Includes bibliography. Electronic reproduction. Boca Raton, Fla., 2012. Mode of access: World Wide Web. |
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| Subject(s): |
Glutamic acids -- Antagonists Cellular signal transduction Glutamic acid -- Metabolism |
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| Persistent Link to This Record: | http://purl.flvc.org/FAU/3342206 | |
| Use and Reproduction: | http://rightsstatements.org/vocab/InC/1.0/ | |
| Host Institution: | FAU |
