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You’ve got to keep ‘em separated: characterizing lagging chromosome prevention in oral cancer cells

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Date Issued:
2013
Summary:
Mitosis is the separation of duplicated chromosomes into two daughter cells in order to create viable offspring. There are many checks in mitosis to ensure the inherited chromosome number is correct. Sometimes, these checkpoints are overcome and daughter cells inherit defects which can lead to cancer. One defect is the appearance of lagging chromosomes, the result of inaccurate chromosomal separation which leads to incorrect chromosome number termed aneuploidy. Aneuploidy is one of the defining traits of cancerous cells. The potential mechanism of lagging chromosomes in the cancerous cell line UPCI:OSCC070 is investigated in this study. siRNA-induced knockdown of KIFC1, a protein that is involved in the centrosomal clustering to prevent multipolar spindles, was used in the cells. Examining both levels of knockdown and time of exposure, we saw that the loss of KIFC1 led to a significant increase in lagging chromosomes, indicating this protein is critical to proper mitotic progression.
Title: You’ve got to keep ‘em separated: characterizing lagging chromosome prevention in oral cancer cells.
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Name(s): Beltran, Rosa Nathalie
Quintyne, Nicholas
Harriet L. Wilkes Honors College
Type of Resource: text
Genre: Thesis
Date Created: Spring 2013
Date Issued: 2013
Publisher: Florida Atlantic University
Place of Publication: Boca Raton, Fla.
Physical Form: pdf
Extent: 30 p.
Language(s): English
Summary: Mitosis is the separation of duplicated chromosomes into two daughter cells in order to create viable offspring. There are many checks in mitosis to ensure the inherited chromosome number is correct. Sometimes, these checkpoints are overcome and daughter cells inherit defects which can lead to cancer. One defect is the appearance of lagging chromosomes, the result of inaccurate chromosomal separation which leads to incorrect chromosome number termed aneuploidy. Aneuploidy is one of the defining traits of cancerous cells. The potential mechanism of lagging chromosomes in the cancerous cell line UPCI:OSCC070 is investigated in this study. siRNA-induced knockdown of KIFC1, a protein that is involved in the centrosomal clustering to prevent multipolar spindles, was used in the cells. Examining both levels of knockdown and time of exposure, we saw that the loss of KIFC1 led to a significant increase in lagging chromosomes, indicating this protein is critical to proper mitotic progression.
Identifier: FA00003505 (IID)
Note(s): Includes bibliography.
Thesis (B.A.)--Florida Atlantic University, Harriet L. Wilkes Honors College, 2013.
Held by: Florida Atlantic University Libraries
Sublocation: FAU Digital Library
Persistent Link to This Record: http://purl.flvc.org/fau/fd/FA00003505
Use and Reproduction: Copyright © is held by the author, with permission granted to Florida Atlantic University to digitize, archive and distribute this item for non-profit research and educational purposes. Any reuse of this item in excess of fair use or other copyright exemptions requires permission of the copyright holder.
Host Institution: FAU

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