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Selected TLR Agonists Act in Synergy to Reprogram DC-NK Cross-talk and Generate Effector T cells in Nicotinic Environment

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Date Issued:
2016
Summary:
The magnitude of immune responses to vaccination is a critical factor in determining protection from diseases. We reported that nicotine disrupts the properties of DCs that are pivotal in the initiation of immune response to vaccines. Here we investigated whether TLR agonist(s) could overcome the effects of nicotine on human DC and DC-NK cross-talk essential for effector T cell generation. nicDC, nicDC-NK, and nicDC-NK-T cultures exposed to TLR agonists were evaluated for expression of costimulatory molecules, cytokines, and intracellular cytokine IFN-g using ELISA and flow cytometry. Our data shows that among the TLR agonists, TLR3 and TLR8/7 synergistically optimized nicDC maturation co-cultured NK cell activation. Importantly, similar to DC-NK, nicDC-NK treated with TLR3 and TLR8/7 and co-cultured with naïve T cells promoted a comparable number of effector T cells. Our data suggest that the addition of appropriate TLR agonist(s) to vaccine formulation could potentially improve the smokers’ immune response to vaccination.
Title: Selected TLR Agonists Act in Synergy to Reprogram DC-NK Cross-talk and Generate Effector T cells in Nicotinic Environment.
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Name(s): Abu-Nuwar, Emily
Tamjidi, Saba
Nouri-Shirazi, Mahyar
Office of Undergraduate Research and Inquiry
Type of Resource: text
Genre: Poster
Date Created: 2016
Date Issued: 2016
Publisher: Florida Atlantic University
Place of Publication: Boca Raton, Florida
Physical Form: application/pdf
Extent: 1 p.
Language(s): English
Summary: The magnitude of immune responses to vaccination is a critical factor in determining protection from diseases. We reported that nicotine disrupts the properties of DCs that are pivotal in the initiation of immune response to vaccines. Here we investigated whether TLR agonist(s) could overcome the effects of nicotine on human DC and DC-NK cross-talk essential for effector T cell generation. nicDC, nicDC-NK, and nicDC-NK-T cultures exposed to TLR agonists were evaluated for expression of costimulatory molecules, cytokines, and intracellular cytokine IFN-g using ELISA and flow cytometry. Our data shows that among the TLR agonists, TLR3 and TLR8/7 synergistically optimized nicDC maturation co-cultured NK cell activation. Importantly, similar to DC-NK, nicDC-NK treated with TLR3 and TLR8/7 and co-cultured with naïve T cells promoted a comparable number of effector T cells. Our data suggest that the addition of appropriate TLR agonist(s) to vaccine formulation could potentially improve the smokers’ immune response to vaccination.
Identifier: FA00005554 (IID)
Subject(s): College students --Research --United States.
Held by: Florida Atlantic University Libraries
Sublocation: Digital Library
Persistent Link to This Record: http://purl.flvc.org/fau/fd/FA00005554
Restrictions on Access: Author retains rights.
Host Institution: FAU