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GC-MS Investigation of Fecal Sterols as Indicators of Pollution
| Title: | GC-MS Investigation of Fecal Sterols as Indicators of Pollution. |
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| Name(s): |
Price, Christopher Louda, J. William |
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| Type of Resource: | text | |
| Genre: | Poster | |
| Date Created: | 2018 | |
| Date Issued: | 2018 | |
| Publisher: | Florida Atlantic University | |
| Place of Publication: | Boca Raton, Florida | |
| Physical Form: | application/pdf | |
| Extent: | 1 p. | |
| Language(s): | English | |
| Abstract/Description: | The goal of this experiment is to perfect the method of trimethylsilyl derivatization of fecal steroids Coprostan-3-one and Coprostanol using pyridine & N,O-bis-(trimethylsilyl)-trifluoroacetamide (BSTFA) containing 1% of Trimethylchlorosilane (TMCS). The extraction method for sediments involves Soxhlet extraction followed by saponification. The extraction of water samples will be done by XAD-2 resin or SPE with C18 cartridges and extracted with MeCl2. The primary instrument of this experiment was a Gas Chromatography- Mass spectroscopy (GC-MS). In addition, a Perkin-Elmer Autosystem GC coupled to a Perkin-Elmer TurboMass Gold mass spectrometer was used, with Helium as the carrier gas. Lastly, this experiment required a computer with Turbomass software version 5.4.2. to analyze samples after derivatization. The results of the GC-MS analysis showed that the methods of derivatization were correct. | |
| Identifier: | FAU_SR00000049 (IID) | |
| Collection: | FAU Student Research Digital Collection | |
| Subject(s): | College students --Research --United States. | |
| Held by: | Florida Atlantic University Libraries | |
| Sublocation: | Digital Library | |
| Persistent Link to This Record: | http://purl.flvc.org/fau/fd/FAU_SR00000049 | |
| Restrictions on Access: | Copyright © is held by the author, with permission granted to Florida Atlantic University to digitize, archive and distribute this item for non-profit research and educational purposes. Any reuse of this item in excess of fair use or other copyright exemptions requires permission of the copyright holder. | |
| Host Institution: | FAU | |
| Is Part of Series: | Florida Atlantic University Digital Library Collections. |
