You are here
Isolation and characterization of novel conopeptides from the marine cone snail
| Title: | Isolation and characterization of novel conopeptides from the marine cone snail: Conus brunneus. |
 146 views 25 downloads |
|---|---|---|
| Name(s): |
Pflueger, Fred C. Florida Atlantic University, Degree grantor Charles E. Schmidt College of Science Department of Chemistry and Biochemistry |
|
| Type of Resource: | text | |
| Genre: | Electronic Thesis Or Dissertation | |
| Issuance: | multipart monograph | |
| Date Issued: | 2008 | |
| Publisher: | Florida Atlantic University | |
| Physical Form: | ||
| Extent: | xv, 169 p. : ill. (some col.) ; 29 cm. | |
| Language(s): | English | |
| Summary: | Cone snails are predatory marine gastropods that use venom for means of predation and defense. This venom is a complex mixture of conopeptides that selectivity binds to ion channels and receptors, giving them a wide range of potential pharmaceutical applications. Conus brunneus is a wide spread Eastern Pacific cone snail species that preys upon worms (vermivorous). Vermivorous cone snails have developed very specific biochemical strategies for the immobilization of their prey and their venom has not been extensively studied to date. The main objective of this dissertation is the characterization of novel conopeptides isolated from Conus brunneus. Chapter 1 is an introduction and background on cone snails and conopeptides. Chapter 2 details the isolation and characterization of a novel P-superfamily conotoxin. Chapter 3 presents the 3D solution structure of the novel P-superfamily conotoxin. Chapter 4 details the isolation and characterization of two novel M-superfamily conotoxins. Chapter 5 covers the use of nano-NMR to characterize a novel P-superfamily conotoxin using nanomole quantities of sample. Chapter 6 is a reprint of a paper published in the Journal of the American Chemical Society in which we combined and implemented techniques developed in the previous chapters to report the presence of D-(Sd(B-Hydroxyvaline in a polypeptide chain. This dissertation contains the first reported work of a P-superfamily structure obtained directly from the crude venom therefore accurately representing native post-translational modifications. In this paper, crude cone snail venom was characterized by: high performance liquid chromatography, nuclear magnetic resonance spectroscopy, nanonuclear magnetic resonance spectroscopy, mass spectrometry, amino acid analysis, Edman degradation sequencing, and preliminary bioassays. | |
| Identifier: | 700044238 (oclc), 3337185 (digitool), FADT3337185 (IID), fau:3821 (fedora) | |
| Note(s): |
by Fred. C. Pflueger. Thesis (Ph.D.)--Florida Atlantic University, 2008. Includes bibliographical references at end of each chapter. |
|
| Subject(s): |
Gastropoda -- Venom Peptides -- Structure Conus Venom |
|
| Held by: | FBoU FABOC | |
| Persistent Link to This Record: | http://purl.flvc.org/FAU/3337185 | |
| Use and Reproduction: | http://rightsstatements.org/vocab/InC/1.0/ | |
| Host Institution: | FAU |
