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Associated bacterial communities of two deep-water sponges
| Title: | Associated bacterial communities of two deep-water sponges. |
 71 views 17 downloads |
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| Name(s): |
Olson, Julie B., creator McCarthy, Peter J., creator |
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| Type of Resource: | text | |
| Genre: | Article | |
| Issuance: | single unit | |
| Date Issued: | 2005 | |
| Publisher: | Inter-Research Science Center | |
| Extent: | 10 p. | |
| Physical Description: | ||
| Language(s): | English | |
| Identifier: | 3350900 (digitool), FADT3350900 (IID), fau:6489 (fedora), 10.3354/ame039047 (doi) | |
| Note(s): |
A combination of approaches was used to examine the bacterial communities associated with 2 deep-water Scleritoderma spp. sponges. Denaturing gradient gel electrophoresis analysis of partial 16S rRNA genes extracted from sponge tissue was used to determine the structure of the sponge-associated bacterial community. Cultivation studies using a variety of marine-based media and medium additions were performed to characterize the cultivable aerobic heterotrophic bacterial community. DNA was extracted from recovered isolates, mixed, and PCR-denaturing gradient gel electrophoresis was performed to demonstrate the differences between the recoverable microorganisms and the entire bacterial community. This manuscript is available at http://www.int-res.com/ and may be cited as: Olson, J. B., & McCarthy, P. J. (2005). Associated bacterial communities of two deep-water sponges. Aquatic Microbial Ecology, 39(1), 47-55. doi:10.3354/ame039047 Florida Atlantic University. Harbor Branch Oceanographic Institute contribution #1591. |
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| Subject(s): |
Sponges Gel electrophoresis Heterotrophic bacteria Host-bacteria relationships |
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| Persistent Link to This Record: | http://purl.flvc.org/FCLA/DT/3350900 | |
| Links: | http://dx.doi.org/10.3354/ame039047 | |
| Restrictions on Access: | ©2005 Inter-Research Science Center | |
| Host Institution: | FAU |
