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AQUEOUS MICRODROPLET GENERATION IN OIL-FREE ENVIRONMENTS

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Date Issued:
2019
Abstract/Description:
Droplet microfluidics generates and manipulates microdroplets in microfluidic devices at high manufacturing efficiency and controllability. Microdroplets have proven effective in biomedical applications such as single-cell analysis, DNA sequencing, protein partitioning and drug delivery. Conventionally, a series of aqueous microdroplets containing biosamples is generated and controlled in an oil environment. One of the critical challenges in this system is that recovery of the aqueous samples from the oil phase is very difficult and often requires expensive and cumbersome post-processing. Also, the low Reynolds (Re) number characteristic of this system results in low throughput of droplet generation. To circumvent challenges and fully utilize microdroplets for practical clinical applications, this research aims to unpack the fundamental physics that governs droplet generation in oil-free systems including an aqueous two-phase system (ATPS) and a high inertial liquid-gas system.
Title: AQUEOUS MICRODROPLET GENERATION IN OIL-FREE ENVIRONMENTS.
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Name(s): Mastiani, Mohammad, author
Kim, Myeongsub, Thesis advisor
Florida Atlantic University, Degree grantor
College of Engineering and Computer Science
Department of Ocean and Mechanical Engineering
Type of Resource: text
Genre: Electronic Thesis Or Dissertation
Date Created: 2019
Date Issued: 2019
Publisher: Florida Atlantic University
Place of Publication: Boca Raton, Fla.
Physical Form: application/pdf
Extent: 148 p.
Language(s): English
Abstract/Description: Droplet microfluidics generates and manipulates microdroplets in microfluidic devices at high manufacturing efficiency and controllability. Microdroplets have proven effective in biomedical applications such as single-cell analysis, DNA sequencing, protein partitioning and drug delivery. Conventionally, a series of aqueous microdroplets containing biosamples is generated and controlled in an oil environment. One of the critical challenges in this system is that recovery of the aqueous samples from the oil phase is very difficult and often requires expensive and cumbersome post-processing. Also, the low Reynolds (Re) number characteristic of this system results in low throughput of droplet generation. To circumvent challenges and fully utilize microdroplets for practical clinical applications, this research aims to unpack the fundamental physics that governs droplet generation in oil-free systems including an aqueous two-phase system (ATPS) and a high inertial liquid-gas system.
Identifier: FA00013332 (IID)
Degree granted: Dissertation (Ph.D.)--Florida Atlantic University, 2019.
Collection: FAU Electronic Theses and Dissertations Collection
Note(s): Includes bibliography.
Subject(s): Microdroplets
Microfluidics
Microfluidic devices
Microfluidics--methods
Held by: Florida Atlantic University Libraries
Sublocation: Digital Library
Persistent Link to This Record: http://purl.flvc.org/fau/fd/FA00013332
Use and Reproduction: Copyright © is held by the author with permission granted to Florida Atlantic University to digitize, archive and distribute this item for non-profit research and educational purposes. Any reuse of this item in excess of fair use or other copyright exemptions requires permission of the copyright holder.
Use and Reproduction: http://rightsstatements.org/vocab/InC/1.0/
Host Institution: FAU
Is Part of Series: Florida Atlantic University Digital Library Collections.