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EVALUATION OF THE CONTRIBUTION METAGENOMIC SHOTGUN SEQUENCING HAS IN ASSESSING POLLUTION SOURCE AND DEFINING PUBLIC HEALTH AND ENVIRONMENTAL RISKS
| Title: | EVALUATION OF THE CONTRIBUTION METAGENOMIC SHOTGUN SEQUENCING HAS IN ASSESSING POLLUTION SOURCE AND DEFINING PUBLIC HEALTH AND ENVIRONMENTAL RISKS. |
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| Name(s): |
Mercer, Brian, author Esiobu, Nawdiuto, Thesis advisor Florida Atlantic University, Degree grantor Department of Biological Sciences Charles E. Schmidt College of Science |
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| Type of Resource: | text | |
| Genre: | Electronic Thesis Or Dissertation | |
| Date Created: | 2020 | |
| Date Issued: | 2020 | |
| Publisher: | Florida Atlantic University | |
| Place of Publication: | Boca Raton, Fla. | |
| Physical Form: | online resource | |
| Extent: | 55 p. | |
| Language(s): | English | |
| Summary: | State-approved membrane filtration (MF) techniques for water quality assessments were contrasted with metagenomic shotgun sequencing (MSS) protocols to evaluate their efficacy in providing precise health-risk indices for surface waters. Using MSS, the relative numerical abundance of pathogenic bacteria, virulence and antibiotic resistance genes revealed the status and potential pollution sources in samples studied. Traditional culture methods (TCM) showed possible fecal contamination, while MSS clearly distinguished between fecal and environmental bacteria contamination sources, and pinpointed actual risks from pathogens. RNA MSS to detect all viable microorganisms and qPCR of fecal biomarkers were used to assess the possible environmental risk between runoff drainage canals and a swamp area with no anthropogenic impact. Results revealed higher levels of pathogenic bacteria, viruses, and virulence and antibiotic resistance genes in the canal samples. The data underscore the potential utility of MSS in precision risk assessment for public and biodiversity health and tracking of environmental microbiome shifts by field managers and policy makers. | |
| Identifier: | FA00013573 (IID) | |
| Degree granted: | Thesis (M.S.)--Florida Atlantic University, 2020. | |
| Collection: | FAU Electronic Theses and Dissertations Collection | |
| Note(s): | Includes bibliography. | |
| Subject(s): |
Metagenomics Public health--Risk assessment Environmental risks Water quality--Evaluation Metagenomics--methods |
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| Held by: | Florida Atlantic University Libraries | |
| Sublocation: | Digital Library | |
| Persistent Link to This Record: | http://purl.flvc.org/fau/fd/FA00013573 | |
| Use and Reproduction: | Copyright © is held by the author with permission granted to Florida Atlantic University to digitize, archive and distribute this item for non-profit research and educational purposes. Any reuse of this item in excess of fair use or other copyright exemptions requires permission of the copyright holder. | |
| Use and Reproduction: | http://rightsstatements.org/vocab/InC/1.0/ | |
| Host Institution: | FAU | |
| Is Part of Series: | Florida Atlantic University Digital Library Collections. |
